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Multi-pathway dysregulation drives dLAN-induced cardiac injury (A) Heatmap of RNA-seq analysis comparing dLAN-exposed and control zebrafish embryos ( n = 5 zebrafish embryos/group). (B) DCFH-DA staining showing elevated <t>reactive</t> <t>oxygen</t> <t>species</t> (ROS) in dLAN-exposed hearts (dashed outline: cardiac region). Scale bars, 500 μm. (C and D) ELISA quantification of serum heart failure biomarkers, including haptoglobin (HP) (C) and B-type natriuretic peptide (BNP)(D), both significantly increased in dLAN-exposed embryos ( n = 3–4 pools of 50 zebrafish embryos/group). (E) Immunofluorescence staining for activated caspase-3 (red) and DAPI (blue) confirming enhanced apoptosis in dLAN-exposed hearts. Scale bars, 500 μm. (F) Confocal images of Tg (myl7:EGFP;lyz: D sRed) hearts showing neutrophil infiltration (red) into the pericardial space (green myocardium) in dLAN-exposed embryos. Scale bars, 250 μm. (C and D) Data are presented as mean ± SEM. Statistical significance was determined by unpaired two-tailed Student’s t test.
Reactive Oxygen Species Assay Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Multi-pathway dysregulation drives dLAN-induced cardiac injury (A) Heatmap of RNA-seq analysis comparing dLAN-exposed and control zebrafish embryos ( n = 5 zebrafish embryos/group). (B) DCFH-DA staining showing elevated <t>reactive</t> <t>oxygen</t> <t>species</t> (ROS) in dLAN-exposed hearts (dashed outline: cardiac region). Scale bars, 500 μm. (C and D) ELISA quantification of serum heart failure biomarkers, including haptoglobin (HP) (C) and B-type natriuretic peptide (BNP)(D), both significantly increased in dLAN-exposed embryos ( n = 3–4 pools of 50 zebrafish embryos/group). (E) Immunofluorescence staining for activated caspase-3 (red) and DAPI (blue) confirming enhanced apoptosis in dLAN-exposed hearts. Scale bars, 500 μm. (F) Confocal images of Tg (myl7:EGFP;lyz: D sRed) hearts showing neutrophil infiltration (red) into the pericardial space (green myocardium) in dLAN-exposed embryos. Scale bars, 250 μm. (C and D) Data are presented as mean ± SEM. Statistical significance was determined by unpaired two-tailed Student’s t test.
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Multi-pathway dysregulation drives dLAN-induced cardiac injury (A) Heatmap of RNA-seq analysis comparing dLAN-exposed and control zebrafish embryos ( n = 5 zebrafish embryos/group). (B) DCFH-DA staining showing elevated <t>reactive</t> <t>oxygen</t> <t>species</t> (ROS) in dLAN-exposed hearts (dashed outline: cardiac region). Scale bars, 500 μm. (C and D) ELISA quantification of serum heart failure biomarkers, including haptoglobin (HP) (C) and B-type natriuretic peptide (BNP)(D), both significantly increased in dLAN-exposed embryos ( n = 3–4 pools of 50 zebrafish embryos/group). (E) Immunofluorescence staining for activated caspase-3 (red) and DAPI (blue) confirming enhanced apoptosis in dLAN-exposed hearts. Scale bars, 500 μm. (F) Confocal images of Tg (myl7:EGFP;lyz: D sRed) hearts showing neutrophil infiltration (red) into the pericardial space (green myocardium) in dLAN-exposed embryos. Scale bars, 250 μm. (C and D) Data are presented as mean ± SEM. Statistical significance was determined by unpaired two-tailed Student’s t test.
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Multi-pathway dysregulation drives dLAN-induced cardiac injury (A) Heatmap of RNA-seq analysis comparing dLAN-exposed and control zebrafish embryos ( n = 5 zebrafish embryos/group). (B) DCFH-DA staining showing elevated <t>reactive</t> <t>oxygen</t> <t>species</t> (ROS) in dLAN-exposed hearts (dashed outline: cardiac region). Scale bars, 500 μm. (C and D) ELISA quantification of serum heart failure biomarkers, including haptoglobin (HP) (C) and B-type natriuretic peptide (BNP)(D), both significantly increased in dLAN-exposed embryos ( n = 3–4 pools of 50 zebrafish embryos/group). (E) Immunofluorescence staining for activated caspase-3 (red) and DAPI (blue) confirming enhanced apoptosis in dLAN-exposed hearts. Scale bars, 500 μm. (F) Confocal images of Tg (myl7:EGFP;lyz: D sRed) hearts showing neutrophil infiltration (red) into the pericardial space (green myocardium) in dLAN-exposed embryos. Scale bars, 250 μm. (C and D) Data are presented as mean ± SEM. Statistical significance was determined by unpaired two-tailed Student’s t test.
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Multi-pathway dysregulation drives dLAN-induced cardiac injury (A) Heatmap of RNA-seq analysis comparing dLAN-exposed and control zebrafish embryos ( n = 5 zebrafish embryos/group). (B) DCFH-DA staining showing elevated <t>reactive</t> <t>oxygen</t> <t>species</t> (ROS) in dLAN-exposed hearts (dashed outline: cardiac region). Scale bars, 500 μm. (C and D) ELISA quantification of serum heart failure biomarkers, including haptoglobin (HP) (C) and B-type natriuretic peptide (BNP)(D), both significantly increased in dLAN-exposed embryos ( n = 3–4 pools of 50 zebrafish embryos/group). (E) Immunofluorescence staining for activated caspase-3 (red) and DAPI (blue) confirming enhanced apoptosis in dLAN-exposed hearts. Scale bars, 500 μm. (F) Confocal images of Tg (myl7:EGFP;lyz: D sRed) hearts showing neutrophil infiltration (red) into the pericardial space (green myocardium) in dLAN-exposed embryos. Scale bars, 250 μm. (C and D) Data are presented as mean ± SEM. Statistical significance was determined by unpaired two-tailed Student’s t test.
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Multi-pathway dysregulation drives dLAN-induced cardiac injury (A) Heatmap of RNA-seq analysis comparing dLAN-exposed and control zebrafish embryos ( n = 5 zebrafish embryos/group). (B) DCFH-DA staining showing elevated <t>reactive</t> <t>oxygen</t> <t>species</t> (ROS) in dLAN-exposed hearts (dashed outline: cardiac region). Scale bars, 500 μm. (C and D) ELISA quantification of serum heart failure biomarkers, including haptoglobin (HP) (C) and B-type natriuretic peptide (BNP)(D), both significantly increased in dLAN-exposed embryos ( n = 3–4 pools of 50 zebrafish embryos/group). (E) Immunofluorescence staining for activated caspase-3 (red) and DAPI (blue) confirming enhanced apoptosis in dLAN-exposed hearts. Scale bars, 500 μm. (F) Confocal images of Tg (myl7:EGFP;lyz: D sRed) hearts showing neutrophil infiltration (red) into the pericardial space (green myocardium) in dLAN-exposed embryos. Scale bars, 250 μm. (C and D) Data are presented as mean ± SEM. Statistical significance was determined by unpaired two-tailed Student’s t test.
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Multi-pathway dysregulation drives dLAN-induced cardiac injury (A) Heatmap of RNA-seq analysis comparing dLAN-exposed and control zebrafish embryos ( n = 5 zebrafish embryos/group). (B) DCFH-DA staining showing elevated <t>reactive</t> <t>oxygen</t> <t>species</t> (ROS) in dLAN-exposed hearts (dashed outline: cardiac region). Scale bars, 500 μm. (C and D) ELISA quantification of serum heart failure biomarkers, including haptoglobin (HP) (C) and B-type natriuretic peptide (BNP)(D), both significantly increased in dLAN-exposed embryos ( n = 3–4 pools of 50 zebrafish embryos/group). (E) Immunofluorescence staining for activated caspase-3 (red) and DAPI (blue) confirming enhanced apoptosis in dLAN-exposed hearts. Scale bars, 500 μm. (F) Confocal images of Tg (myl7:EGFP;lyz: D sRed) hearts showing neutrophil infiltration (red) into the pericardial space (green myocardium) in dLAN-exposed embryos. Scale bars, 250 μm. (C and D) Data are presented as mean ± SEM. Statistical significance was determined by unpaired two-tailed Student’s t test.
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Multi-pathway dysregulation drives dLAN-induced cardiac injury (A) Heatmap of RNA-seq analysis comparing dLAN-exposed and control zebrafish embryos ( n = 5 zebrafish embryos/group). (B) DCFH-DA staining showing elevated reactive oxygen species (ROS) in dLAN-exposed hearts (dashed outline: cardiac region). Scale bars, 500 μm. (C and D) ELISA quantification of serum heart failure biomarkers, including haptoglobin (HP) (C) and B-type natriuretic peptide (BNP)(D), both significantly increased in dLAN-exposed embryos ( n = 3–4 pools of 50 zebrafish embryos/group). (E) Immunofluorescence staining for activated caspase-3 (red) and DAPI (blue) confirming enhanced apoptosis in dLAN-exposed hearts. Scale bars, 500 μm. (F) Confocal images of Tg (myl7:EGFP;lyz: D sRed) hearts showing neutrophil infiltration (red) into the pericardial space (green myocardium) in dLAN-exposed embryos. Scale bars, 250 μm. (C and D) Data are presented as mean ± SEM. Statistical significance was determined by unpaired two-tailed Student’s t test.

Journal: iScience

Article Title: Dim light at night induces cardiac injury in zebrafish embryos via disrupted chloride homeostasis

doi: 10.1016/j.isci.2026.115796

Figure Lengend Snippet: Multi-pathway dysregulation drives dLAN-induced cardiac injury (A) Heatmap of RNA-seq analysis comparing dLAN-exposed and control zebrafish embryos ( n = 5 zebrafish embryos/group). (B) DCFH-DA staining showing elevated reactive oxygen species (ROS) in dLAN-exposed hearts (dashed outline: cardiac region). Scale bars, 500 μm. (C and D) ELISA quantification of serum heart failure biomarkers, including haptoglobin (HP) (C) and B-type natriuretic peptide (BNP)(D), both significantly increased in dLAN-exposed embryos ( n = 3–4 pools of 50 zebrafish embryos/group). (E) Immunofluorescence staining for activated caspase-3 (red) and DAPI (blue) confirming enhanced apoptosis in dLAN-exposed hearts. Scale bars, 500 μm. (F) Confocal images of Tg (myl7:EGFP;lyz: D sRed) hearts showing neutrophil infiltration (red) into the pericardial space (green myocardium) in dLAN-exposed embryos. Scale bars, 250 μm. (C and D) Data are presented as mean ± SEM. Statistical significance was determined by unpaired two-tailed Student’s t test.

Article Snippet: Reactive oxygen species Assay Kit , Nanjing Jiancheng Bioengineering Institute , Cat# E004-1-1.

Techniques: RNA Sequencing, Control, Staining, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Two Tailed Test